ABSTRACT
This study was aimed to prepare the polyclonal antibody against the soluble proliferation-inducing ligand (sAPRIL) antigen and to investigate its effects in suppressing sAPRIL mediated lymphocyte proliferation. Mutated recombinant sAPRIL protein, which lacks biological activity but maintains immunogenicity, was used as antigen to immunize humanized SCID mice. Sera were obtained at 6 weeks after immunization. Indirect ELISA and Western blot were used to detect the antibody titer and specificity. The inhibition of polyclonal antibodies on Raji and Jurkat cell proliferation stimulated by sAPRIL was assessed by the MTT assay. The results showed that the mutant of sAPRIL could induce the production of polyclonal antibodies against human sAPRIL. Western blot and indirect ELISA analyses indicated that the anti-serum had higher specificity with a titer of 1:640. Functional analysis revealed that these polyclonal antibodies significantly inhibited the proliferation of Raji and Jurkat cell stimulated by sAPRIL (p < 0.05). It is concluded the polyclonal antibody against human sAPRIL is successfully prepared, which can inhibit the proliferation of Raji and Jurkat cells stimulated by sAPRIL in vitro.
Subject(s)
Animals , Humans , Mice , Antibodies , Genetics , Allergy and Immunology , Pharmacology , Antibody Specificity , Allergy and Immunology , Cell Proliferation , Cloning, Molecular , Immune Sera , Allergy and Immunology , Jurkat Cells , Tumor Necrosis Factor Ligand Superfamily Member 13 , Genetics , Allergy and ImmunologyABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of intense pulsed light (IPL) on transforming growth factor-beta1 mRNA (TGF-beta1 mRNA) expression in rat skin and explore the molecular mechanisms of photorejuvenation.</p><p><b>METHODS</b>Fifteen SD rats were exposed to IPL in 3 dermal regions with triple pulses (duration of 4, 5, and 6 ms) at the energy density of 34 J/cm2 and pulse delay of 20 or 25 ms. On days 1, 3, 5, 7, 15, and 30 after the treatment, skin specimens from the treated and non-treated areas were obtained to detect TGF-beta1 mRNA expression with in situ hybridization.</p><p><b>RESULTS</b>In the UPL-exposed skin areas, TGF-beta1 mRNA expression was detected in the epidermal keratinocytes and dermal cells 1 day after the exposure, reaching the highest expression level on day 7 followed by gradual decrement since day 15, and till day 30, only weak expression was found in the dermal cells. In the non-exposed regions, the cells remained negative for TGF-beta1 mRNA.</p><p><b>CONCLUSION</b>IPL can enhance TGF-beta1 mRNA expression in the skin, suggesting that TGF-beta1 plays an important role in dermal remodeling in photorejuvenation.</p>
Subject(s)
Animals , Female , Male , Rats , Phototherapy , Methods , RNA, Messenger , Genetics , Metabolism , Random Allocation , Rats, Sprague-Dawley , Rejuvenation , Skin , Metabolism , Radiation Effects , Transforming Growth Factor beta1 , Genetics , Metabolism , Radiation EffectsABSTRACT
<p><b>OBJECTIVE</b>To investigate a new method of genioplasty for treatment of a prominent mandibular angle with mandibular malformation.</p><p><b>METHODS</b>According to the different types of the mandible, the prominent mandibular angle was grinded with the masseter muscle kept intact. Autologous micromorselized bone of the grinded mandibular angle was collected and implanted into the chin for augmentation mentoplasty, and the chin was fixed with gypsum after operation.</p><p><b>RESULTS</b>Fifteen patients receiving the surgery were followed up for 6 -12 months, and satisfactory effects were achieved in 12 patients. One patient requested modification of the shape of the chin 10 days after the operation, and another two sustained chin distortion due to external forces and underwent subsequent shape adjustment of the chin.</p><p><b>CONCLUSION</b>The procedure described avoids graft rejection and causes less complications for genioplasty with easy moulding and satisfactory outcome.</p>